Glucose metabolism in murine hybridoma cells.

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ContributionsManchester Polytechnic. Department of Biological Sciences.
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Open LibraryOL16582374M

Christie A, Butler M. Growth and metabolism of a murine hybridoma in cultures containing glutamine-based dipeptides. Focus. ; – Chuppa S, Tsai Y-S, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D.

Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian by: The results of these preliminary studies, especially the kinetic model for the metabolism of immobilized cells were applied to a laboratory scale reactor (fig.

2).The reactor consists of two chambers (outer chamber 1, inner chamber 1) separated by a dialysis membrane (cuprophan, Enka, Germany) with a cut-off of Dalton [3].In the inner chamber a radial-flow fixed bed (volume The energy metabolism of murine hybridomas, designated as low and high antibody Glucose metabolism in murine hybridoma cells.

book, was investigated to determine the pattern of energy metabolism during cellular productivity. The maximum specific activities of regulatory enzymes of glutaminolysis, glycolysis and the TCA cycle changed significantly during the course of batch by: 2. The energy metabolism of a mammalian cell line grown in vitro was analyzed by substrate consumption rates and metabolic flux measurements.

The data allowed the determination of the relative importance of the pathways of glucose and glutamine metabolism to the energy requirements of the cell.

Details Glucose metabolism in murine hybridoma cells. FB2

Changes in the substrate concentrations during culture contributed to the changing catalytic activities Cited by: Metabolic flux rewiring is associated with altered expression and activity of metabolic enzymes. Although the shift to aerobic glycolysis has been a scientific paradox—and a stumbling block of the mammalian biotech industry—for some time, the underlying molecular alterations associated with metabolic flux rewiring in CCLs have been largely by: Modification of glucose and glutamine metabolism in hybridoma cells through metabolic engineering.

Authors; A maximum reduction of about 47% in the glucose consumption rate was obtained for one of the transformations. However a main drawback was the lack of stability of the transformed cells Growth and metabolism of a murine hybridoma Cited by: Effects of glucose and glutamine on metabolism and antibody production were investigated in continuous culture of hybridoma cells using a serum-free medium.

Viable cell concentration remained. Metabolism (/ m ə ˈ t æ b ə l ɪ z ə m /, from Greek: μεταβολή metabolē, "change") is the set of Glucose metabolism in murine hybridoma cells. book chemical reactions in three main purposes of metabolism are: the conversion of food to energy to run cellular processes; the conversion of food/fuel to building blocks for proteins, lipids, nucleic acids, and some carbohydrates; and the elimination of.

Anthony Mancuso, Susan T. Sharfstein, Erik J. Fernandez, Douglas S. Clark and Harvey W. Blanch, Effect of extracellular glutamine concentration on primary and secondary metabolism of a murine hybridoma: An in vivo 13C nuclear magnetic resonance study, Biotechnology and Bioengineering, 57, 2, (), ().Cited by:   In vivo effect of metformin on tumor glucose consumption and cancer growth.

CT26 and 4T1 cells were subcutaneously inoculated in the hip of Cited by: Carbohydrate metabolism is the whole of the biochemical processes responsible for the metabolic formation, breakdown, and interconversion of carbohydrates in living organisms.

Carbohydrates are central to many essential metabolic pathways. Plants synthesize carbohydrates from carbon dioxide and water through photosynthesis, allowing them to store energy absorbed from sunlight internally.

Glucose metabolism supplies energy for the growth and maintainence of mammalian cells (7), and cancer cells are well-known to employ glycolysis for energy metabolism (8, 9). As soon as the primary.

Glucose Metabolism study guide by cmorg includes 20 questions covering vocabulary, terms and more. Quizlet flashcards, activities and games help you improve your grades.

Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular Cited by: Fig.

Regulation of cancer metabolism pathways by oncogenes and tumor suppressors. Metabolic enzymes are regulated by oncogenes – Myc, Akt, and receptor tyrosine kinases (RTKs) – and the tumor suppressors 5′ adenosine monophosphate-activated protein kinase (AMPK) and pKey metabolic pathways are represented within colored boxes: blue indicates pathways linked to glucose metabolism.

Hybridomas are finding increased use for the production of a wide variety of monoclonal antibodies. Understanding the roles of physiological and environmental factors on the growtCited by:   Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress.

Nat. Commun. 8, doi: /ncomms ().Cited by: Glucose metabolism in cancer cells Alessandro Annibaldi and Christian Widmann Introduction Carcinogenesis is a complex, multistep process that requires the elimination of several cell-imposed barriers such as antiproliferative responses, programmed cell death-inducing mechanisms, and senescence.

This occurs mostly through mutations in oncogenes File Size: KB. • is present in the liver, pancreas, small intestine. Km =20mM => is not saturated by the blood level of glucose (mM), • serves as a glucose sensor in the beta cells of the pancreatic islets to control insulin release.

Similarly, it controls glucagon release in the alpha pancreatic cells. Monoclonal Antibodies in Biotechnology; Monoclonal Antibodies in Biotechnology. Monoclonal Antibodies in Biotechnology Theoretical and Practical Aspects.

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Description Glucose metabolism in murine hybridoma cells. EPUB

Log in Register Recommend to librarianCited by:   A number of studies reported the effects of ammonia on cell metabolism. For a murine hybridoma cell line, Ozturk et al. (6) observed a roughly twofold increase in glucose and glutamine consumption rates in cultures exposed to mM of ammonia, compared to control conditions (Fig.

3)/5. Animal cell technology has undergone a rapid transformation over the last decade from a research tool and highly specialised technology to a central resource for innovation in pharmaceutical research and development.

These proceedings of the 14th Meeting of the European Society for Animal Cell. glucose metabolism to provide cellular fuel. This augmented glucose metabolism is highly regulated and has a profound impact on a sustained antiviral response.

Thus, impaired glucose metabolism in T cells has been demonstrated to prevent full effector function, with failure of T cells to proliferate and to maximally express cytokines.

Energy obtained from metabolism (e.g. oxidation of glucose) is usually stored temporarily within cells in the form of ATP. Organisms capable of aerobic. To select hybridoma cells, the pool of cells resulting from the fusion (a mix of hybridoma cells and non-fused B lymphocytes and myeloma cells) are cultivated in a selective medium containing aminopterin, which inhibits the nucleotide de novo synthesis.

Myeloma cells lack the salvage pathway for nucleotide production. When they are exposed to Cited by: 2. will like to investigate on the hybridoma cells requirement of glucose and glutamine for growth rate and also the effect of the by-products to the growth rate of the cell.

Previous study showed that the researcher uses a glucose and glutamine concentration from – 24 mM and 4 – 12 mM batch culture. Cancer cells need food to survive and grow.

They're very good at getting it, too, even when nutrients are scarce. Many scientists have tried killing. It is hard to begin a discussion of cancer cell metabolism without first mentioning Otto Warburg.

A pioneer in the study of respiration, Warburg made a striking dis - covery in the s. He found that, even in the presence of ample oxygen, cancer cells prefer to metabolize glucose by gly.

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It fond that metabolites of glycolysis and the citric acid cycle such as fructore-1,6-bisphosphate, phosphoenolpyruvate, 3-phosphoglycerate, citrate and oxaloacetate significantly enhance insulin exocytosis from pancreatic β-cells by inactivating ser/thr phosphatases.

Abnormal glucose metabolism, which occurs when the body has difficulty processing sugar (glucose) into energy, is twice as common among patients with chronic nerve dysfunction of unknown cause. Killing cancer cells by targeting glucose metabolism inhibition of tumor metabolism is a promising new therapeutic approach.

However, little is known .Major Pathways in Carbohydrate Metabolism In animals, excess glucose is converted to its storage form, glycogen, by glycogenesis. When glucose is needed as a source of energy or as a precursor molecule in biosynthetic processes, glycogen is degraded by glycogenolysis.

Glucose can .Ffluorodeoxyglucose is an analog of glucose which is taken up by cells to the same extent as glucose, but it is not metabolized.

Because of these findings the Tumor Glucose Metabolism Study is capable of demonstrating primary and metastatic cancer throughout the body. Indications: 2 - File Size: KB.